Guided nuclease vector infection
Integration, transcription
Successful cassette expression
Donor plasmid co-transfection
Double strand break at the target locus
Correcting the reading frame
By homology-directed repair
A functional allele, recovered
Of ashes and bones
Bereft, yet flooded and drowned
By the cold caress of the midnight tides
Ending its journey through time
Now the water has eroded the stone
Washing away all those ashes and bones
Let the river weep its own course
Bleeding from the mountainside walls
Rejoice!
Embrace this transformation!